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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM10 Recombinant antibody (84104-4-RR)

    WB analysis using 84104-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84104-4-RR (RBM10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RBM10 Recombinant antibody (84104-4-RR)

    IHC staining of mouse brain using 84104-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RBM10 Recombinant antibody (84104-4-RR)

    IHC staining of mouse brain using 84104-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RBM10 Recombinant antibody (84104-4-RR)

    IHC staining of human placenta using 84104-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RBM10 Recombinant antibody (84104-4-RR)

    IHC staining of human placenta using 84104-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RBM10 Recombinant antibody (84104-4-RR)

    IF Staining of A431 using 84104-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM10 antibody (84104-4-RR, Clone: 241151G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84104-4-RR

    Affinity and Kinetic Characterization of 84104-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84104-4-RR against Human RBM10 were performed. The affinity constant is 0.33 nM.

RBM10 Recombinant monoclonal antibody

RBM10 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84104-4-RR
Clone No.241151G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, MDA-MB-231 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84104-4-RR targets RBM10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5788

Product name: Recombinant human RBM10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 601-930 aa of BC024153

Sequence: QSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTKTAQQIAKDMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAGYAILEKKGALAERQHTSMDLPKLASDDRPSPPRGLVAAYSGESDSEEEQERGGPEREEKLTDWQKLACLLCRRQFPSKEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDMEQMKYRDRAAERREKYGIPEPPEPKRRKYGGISTASVDFEQPTRDGLGSDNIGSRMLQAMGWKEGSGLGRKKQGIVTPIEAQTRVRGSGLGARGSSYGVTSTESYKETLHKTMVTRFNEAQ

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 10
Calculated molecular weight 104 kDa
Observed molecular weight100-120 kDa
GenBank accession numberBC024153
Gene Symbol RBM10
Gene ID (NCBI) 8241
RRIDAB_3671671
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP98175
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

Protocols

Product Specific Protocols
IF protocol for RBM10 antibody 84104-4-RRDownload protocol
IHC protocol for RBM10 antibody 84104-4-RRDownload protocol
WB protocol for RBM10 antibody 84104-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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