Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 11 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
14423-1-AP targets RBM10 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5788 Product name: Recombinant human RBM10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 601-930 aa of BC024153 Sequence: QSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTKTAQQIAKDMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAGYAILEKKGALAERQHTSMDLPKLASDDRPSPPRGLVAAYSGESDSEEEQERGGPEREEKLTDWQKLACLLCRRQFPSKEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDMEQMKYRDRAAERREKYGIPEPPEPKRRKYGGISTASVDFEQPTRDGLGSDNIGSRMLQAMGWKEGSGLGRKKQGIVTPIEAQTRVRGSGLGARGSSYGVTSTESYKETLHKTMVTRFNEAQ 相同性解析による交差性が予測される生物種 |
| Full Name | RNA binding motif protein 10 |
| Calculated molecular weight | 104 kDa |
| Observed molecular weight | 115 kDa |
| GenBank accession number | BC024153 |
| Gene Symbol | RBM10 |
| Gene ID (NCBI) | 8241 |
| RRID | AB_2175593 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98175 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RBM10 antibody 14423-1-AP | Download protocol |
| IP protocol for RBM10 antibody 14423-1-AP | Download protocol |
| WB protocol for RBM10 antibody 14423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A RNA-binding motif protein 10 inactivates c-Myc by partnering with ribosomal proteins uL18 and uL5
| ||
Oncogene RNA-binding motif protein 10 induces apoptosis and suppresses proliferation by activating p53.
| ||
Exp Cell Res RBM10 regulates the tumorigenic potential of human cancer cells by modulating PPM1B and YBX1 activities
| ||
BMC Mol Biol Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA.
| ||
J Cell Mol Med RBM10 inhibits cell proliferation of lung adenocarcinoma via RAP1/AKT/CREB signalling pathway. | ||
Oxid Med Cell Longev RBM10 Is a Biomarker Associated with Pan-Cancer Prognosis and Immune Infiltration: System Analysis Combined with In Vitro and Vivo Experiments
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