Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31590-1-AP targets RBKS in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36374 Product name: Recombinant human RBKS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-322 aa of BC017425 Sequence: MAASGEPQRQWQEEVAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF 相同性解析による交差性が予測される生物種 |
| Full Name | ribokinase |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC017425 |
| Gene Symbol | RBKS |
| Gene ID (NCBI) | 64080 |
| RRID | AB_3670048 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H477 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBKS also known as ribokinase, is a member of the carbohydrate kinase PfkB family. The protein encoded by this gene phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium, which is the first step in ribose metabolism. This process is crucial for the metabolism of ribose, a sugar that is a component of RNA and ATP. In summary, RBKS is an important gene involved in the initial step of ribose metabolism, with its protein product playing a key role in phosphorylating ribose to ribose-5-phosphate, a reaction that is essential for further metabolic processes involving this sugar.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RBKS antibody 31590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |