Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, COS-7 cells, HEK-293T cells, HeLa cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
Product Information
20365-1-AP targets RbAp46 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | retinoblastoma binding protein 7 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | NM_002893 |
| Gene Symbol | RBBP7 |
| Gene ID (NCBI) | 5931 |
| RRID | AB_10697660 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16576 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| IHC protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| IF protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| IP protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Gastroenterol Hepatol Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.
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Oncogene HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer. | ||
Cancer Cell Int Platelet-activating factor acetyl hydrolase IB2 dysregulated cell proliferation in ovarian cancer. | ||
Biochem Biophys Res Commun PAFAH1B2 is a HIF1a target gene and promotes metastasis in pancreatic cancer.
| ||
Oncol Lett RBBP7 is a prognostic biomarker in patients with esophageal squamous cell carcinoma.
| ||
Oncogene ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression |