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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBBP6 Polyclonal antibody (11882-1-AP)

    WB analysis using 11882-1-AP (same clone as 11882-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11882-1-AP (RBBP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBBP6 Polyclonal antibody (11882-1-AP)

    WB analysis using 11882-1-AP (same clone as 11882-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11882-1-AP (RBBP6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RBBP6 Polyclonal antibody (11882-1-AP)

    IHC staining of human placenta using 11882-1-AP (same clone as 11882-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11882-1-AP (RBBP6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using RBBP6 Polyclonal antibody (11882-1-AP)

    IHC staining of human rectal cancer using 11882-1-AP (same clone as 11882-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 11882-1-AP (RBBP6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RBBP6 Polyclonal antibody (11882-1-AP)

    IHC staining of mouse kidney using 11882-1-AP (same clone as 11882-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11882-1-AP (RBBP6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RBBP6 Polyclonal antibody, PBS Only

RBBP6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11882-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase RBBP6, EC:2.3.2.27, MY038, P2P R, P2PR

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11882-1-PBS targets RBBP6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2484

Product name: Recombinant human RBBP6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-118 aa of BC029352

Sequence: MSCVHYKFSSKLNYDTVTFDGLHISLCDLKKQIMGREKLKAADCDLQITNAQTKEEYTDDNALIPKNSSVIVRRIPIGGVKSTSKTYVISRTEPAMATTKAVCKNTISHFFYTLLLPL

相同性解析による交差性が予測される生物種
Full Name retinoblastoma binding protein 6
Calculated molecular weight 1792 aa, 200 kDa
Observed molecular weight250 kDa
GenBank accession numberBC029352
Gene Symbol RBBP6
Gene ID (NCBI) 5930
RRIDAB_2177794
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6E9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].

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