Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 14557-1-AP (same clone as 14557-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 14557-1-AP (same clone as 14557-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
WB analysis of mouse thymus using 14557-1-AP (same clone as 14557-1-PBS)
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 14557-1-AP (same clone as 14557-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 14557-1-AP (same clone as 14557-1-PBS)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 14557-1-AP (same clone as 14557-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBAP48 antibody (14557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14557-1-PBS in a different storage buffer formulation.

RBAP48 Polyclonal antibody, PBS Only

RBAP48 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14557-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RBBP4, NURF55, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14557-1-PBS targets RBAP48 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6110 Product name: Recombinant human RBBP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-425 aa of BC053904 Sequence: MADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLVFDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHTICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIGEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMAENIYNDEDPEGSVDPEGQGS 相同性解析による交差性が予測される生物種
Full Name	retinoblastoma binding protein 4
Calculated molecular weight	48 kDa
Observed molecular weight	40 kDa, 48-53 kDa
GenBank accession number	BC053904
Gene Symbol	RBBP4
Gene ID (NCBI)	5928
RRID	AB_2177630
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q09028
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 14557-1-AP (same clone as 14557-1-PBS)

WB analysis of K-562 using 14557-1-AP (same clone as 14557-1-PBS)

WB analysis of mouse thymus using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human ovary using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human ovary using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human kidney using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human kidney using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human placenta using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human placenta using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human testis using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human testis using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human brain using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human brain using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human spleen using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human spleen using 14557-1-AP (same clone as 14557-1-PBS)

IHC staining of human lung using 14557-1-AP (same clone as 14557-1-PBS)