Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, mouse brain tissue, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 18 publications below |
| IHC | See 3 publications below |
| IF | See 7 publications below |
| IP | See 1 publications below |
Product Information
10043-2-AP targets RB1CC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | RB1-inducible coiled-coil 1 |
| Calculated molecular weight | 1594 aa, 183 kDa |
| Observed molecular weight | 180-200 kDa |
| GenBank accession number | BC017556 |
| Gene Symbol | RB1CC1 |
| Gene ID (NCBI) | 9821 |
| RRID | AB_2253571 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDY2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation[PMID:15968549 ]. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation [PMID: 19258318]. It is probably involved in the tumorigenesis of breast cancer[PMID: 14533007]. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene[PMID: 21602932]. The immunogen of this antibody is C-terminal 154aa of RB1CC1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RB1CC1 antibody 10043-2-AP | Download protocol |
| IHC protocol for RB1CC1 antibody 10043-2-AP | Download protocol |
| IF protocol for RB1CC1 antibody 10043-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Immunol Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.
| ||
Nat Cell Biol mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6. | ||
Nat Struct Mol Biol Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and -independent autophagy. |