Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, A431 cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
25628-1-AP targets RB1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RB1 fusion protein Ag22578 相同性解析による交差性が予測される生物種 |
| Full Name | retinoblastoma 1 |
| Calculated molecular weight | 928 aa, 106 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC039060 |
| Gene Symbol | RB1 |
| Gene ID (NCBI) | 5925 |
| RRID | AB_2880168 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06400 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RB1 antibody 25628-1-AP | Download protocol |
| IF protocol for RB1 antibody 25628-1-AP | Download protocol |
| IP protocol for RB1 antibody 25628-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Artif Cells Nanomed Biotechnol Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway. | ||
Oncol Rep Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma. | ||
Oncol Lett 14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells. | ||
Cell Death Dis MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis. | ||
Biomed Pharmacother Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo. |