Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, K-562 cells, Raji cells, Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29510-1-AP targets RASGRP4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31219 Product name: Recombinant human RASGRP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 108-170 aa of BC146669 Sequence: KATGDTQELRRLQICHLVRYWLMRHPEVMHQDPQLEEVIGRFWATVAREGNSAQRRLGDSSDL 相同性解析による交差性が予測される生物種 |
| Full Name | RAS guanyl releasing protein 4 |
| Calculated molecular weight | 673 aa, 75 kDa |
| Observed molecular weight | 54-75 kDa |
| GenBank accession number | BC146669 |
| Gene Symbol | RASGRP4 |
| Gene ID (NCBI) | 115727 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDF6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RASGRP4 is a member of the RAS guanine nucleotide-releasing protein (RASGRP) family, which are crucial guanine nucleotide exchange factors (GEFs). The primary function of this family is to act as molecular switches that activate RAS signaling pathways by promoting the exchange of GDP for GTP on small GTPases. Specifically, RASGRP4 is highly and preferentially expressed in mast cells. It plays a vital role in the development, maturation, and functional activation of these immune cells by regulating key signaling cascades, such as the MAPK pathway.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RASGRP4 antibody 29510-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |