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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using RASGEF1B Polyclonal antibody (13617-1-AP)

    WB analysis of human brain using 13617-1-AP (same clone as 13617-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13617-1-AP (RASGEF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13617-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using RASGEF1B Polyclonal antibody (13617-1-AP)

    WB analysis of mouse lung using 13617-1-AP (same clone as 13617-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13617-1-AP (RASGEF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13617-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using RASGEF1B Polyclonal antibody (13617-1-AP)

    WB analysis of mouse ovary using 13617-1-AP (same clone as 13617-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13617-1-AP (RASGEF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13617-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RASGEF1B Polyclonal antibody (13617-1-AP)

    IHC staining of human stomach using 13617-1-AP (same clone as 13617-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach using 13617-1-AP (RASGEF1B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13617-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RASGEF1B Polyclonal antibody (13617-1-AP)

    IHC staining of human stomach using 13617-1-AP (same clone as 13617-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach using 13617-1-AP (RASGEF1B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13617-1-PBS in a different storage buffer formulation.

RASGEF1B Polyclonal antibody, PBS Only

RASGEF1B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13617-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13617-1-PBS targets RASGEF1B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4525

Product name: Recombinant human RASGEF1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of BC036784

Sequence: MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDRTYIFTFLLSSRLFMHPYELMAKVCHLCVEHQRLSDPDSDKNQMRKIAPKILQLLTEWTETFPYDFRDERMMRNLKDLAHRIASGEEVGNLNLARLLEFPGRAWVGNGAELCILISTASSLFCLWASPPHHILVYLVCQVRMIIPSHFKGI

相同性解析による交差性が予測される生物種
Full Name RasGEF domain family, member 1B
Calculated molecular weight 55 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC036784
Gene Symbol RASGEF1B
Gene ID (NCBI) 153020
RRIDAB_10644147
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VAM2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RASGEF1B is a guanine-nucleotide exchange factor, whose expression is induced in macrophages on stimulation by TLR2 and TLR4 agonists. It interacts with Ras family proteins. It also interacts with CCDC124 during cytokinesis.

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