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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using RASEF Monoclonal antibody (67159-1-Ig)

    WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

    WB result of RASEF antibody (67159-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RASEF transfected LNCaP cells. This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using RASEF Monoclonal antibody (67159-1-Ig)

    WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67159-1-Ig (RASEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of mouse pancreas using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of mouse pancreas using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human ovary tumor using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human ovary tumor using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human pancreas cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human pancreas cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RASEF Monoclonal antibody (67159-1-Ig)

    IF Staining of A431 using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASEF antibody (67159-1-Ig, Clone: 1C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RASEF Monoclonal antibody, PBS Only

RASEF Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67159-1-PBS
Clone No.1C2B2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RAB45, Ras and EF-hand domain-containing protein, Ras related protein Rab 45, Ras-related protein Rab-45

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67159-1-PBS targets RASEF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag2149

Product name: Recombinant human RASEF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 354-740 aa of BC023566

Sequence: DGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

相同性解析による交差性が予測される生物種
Full Name RAS and EF-hand domain containing
Calculated molecular weight 740 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC023566
Gene Symbol RASEF
Gene ID (NCBI) 158158
RRIDAB_2882456
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8IZ41
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.

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