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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RASEF Polyclonal antibody (28162-1-AP)

    WB analysis using 28162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28162-1-AP (RASEF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RASEF Polyclonal antibody (28162-1-AP)

    IHC staining of human stomach using 28162-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28162-1-AP (RASEF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using RASEF Polyclonal antibody (28162-1-AP)

    IHC staining of human stomach using 28162-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28162-1-AP (RASEF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using RASEF Polyclonal antibody (28162-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 28162-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28162-1-AP (RASEF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using RASEF Polyclonal antibody (28162-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 28162-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28162-1-AP (RASEF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RASEF Polyclonal antibody

RASEF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28162-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

Ras-related protein Rab-45, Ras related protein Rab 45, Ras and EF-hand domain-containing protein, RAB45

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, PC-12 cells
Positive IHC detected inhuman stomach tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28162-1-AP targets RASEF in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28049

Product name: Recombinant human RASEF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-245 aa of BC023566

Sequence: MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGD

相同性解析による交差性が予測される生物種
Full Name RAS and EF-hand domain containing
Calculated molecular weight 740 aa, 83 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC023566
Gene Symbol RASEF
Gene ID (NCBI) 158158
RRIDAB_2881079
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZ41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RASEF, or RAS and EF-hand domain-containing protein, is a member of the Rab GTPase protein family. RASEF is a protein with significant implications in lung cancer, potentially serving as both a prognostic biomarker and a therapeutic target. Its role in cancer cell growth and its interaction with ERK1/2 signaling make it a promising candidate for further research and development of new molecular therapies in lung cancer treatment.

Protocols

Product Specific Protocols
IHC protocol for RASEF antibody 28162-1-APDownload protocol
WB protocol for RASEF antibody 28162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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