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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RASAL2 Polyclonal antibody (22140-1-AP)

    WB analysis of HeLa using 22140-1-AP (same clone as 22140-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RASAL2 Polyclonal antibody (22140-1-AP)

    IF Staining of HeLa using 22140-1-AP (same clone as 22140-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22140-1-AP (RASAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RASAL2 Polyclonal antibody, PBS Only

RASAL2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 22140-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22140-1-PBS targets RASAL2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17555

Product name: Recombinant human RASAL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 609-958 aa of BC110611

Sequence: LRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDERESSLPNGRSVSLMDLQDTHAAQVEHASVMLDVPIRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGGLQPLSFQNPVYHLNNPIPAMPKASIDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFT

相同性解析による交差性が予測される生物種
Full Name RAS protein activator like 2
Calculated molecular weight 1262 aa, 142 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC110611
Gene Symbol RASAL2
Gene ID (NCBI) 9462
RRIDAB_2879005
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJF2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RASAL2 (Ras protein activator like 2), also known as nGAP, is a Ras GTPase-activating protein, containing a GRD domain and a coiled-coil structure at the C terminus. It is able to complement the defective RasGAP function in a yeast system. RASAL2 is highly homologous to Ras-specific GTPase activating proteins (RasGAPs). Two transcript variants exist for RASAL2 due to alternative splicing events.

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