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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RASAL2 Polyclonal antibody (22140-1-AP)

    WB analysis of HeLa using 22140-1-AP (same clone as 22140-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RASAL2 Polyclonal antibody (22140-1-AP)

    IHC staining of mouse testis using 22140-1-AP (same clone as 22140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using RASAL2 Polyclonal antibody (22140-1-AP)

    IHC staining of rat testis using 22140-1-AP (same clone as 22140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RASAL2 Polyclonal antibody (22140-1-AP)

    IHC staining of human testis using 22140-1-AP (same clone as 22140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RASAL2 Polyclonal antibody (22140-1-AP)

    IF Staining of HeLa using 22140-1-AP (same clone as 22140-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22140-1-AP (RASAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RASAL2 Polyclonal antibody, PBS Only

RASAL2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 22140-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Ras GTPase activating protein nGAP, Ras GTPase-activating protein nGAP, RAS protein activator like 2, RAS protein activator-like 2, RASAL 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22140-1-PBS targets RASAL2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17555

Product name: Recombinant human RASAL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 609-958 aa of BC110611

Sequence: LRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDERESSLPNGRSVSLMDLQDTHAAQVEHASVMLDVPIRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGGLQPLSFQNPVYHLNNPIPAMPKASIDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFT

相同性解析による交差性が予測される生物種
Full Name RAS protein activator like 2
Calculated molecular weight 1262 aa, 142 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC110611
Gene Symbol RASAL2
Gene ID (NCBI) 9462
RRIDAB_2879005
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJF2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RASAL2 (RAS protein activator like 2) is a protein-coding gene that encodes a member of the RAS GTPase-activating protein (RAS-GAP) family. The encoded protein contains a characteristic GAP-related domain (GRD) that enables it to catalyze the hydrolysis of RAS-GTP to RAS-GDP, thereby functioning as a negative regulator of RAS signaling. Structurally, RASAL2 also possesses additional domains including a Pleckstrin homology (PH) domain and a C2 domain, which are involved in membrane localization and calcium/lipid binding. RASAL2 has been extensively studied for its context-dependent roles in cancer, predominantly functioning as a tumor suppressor in malignancies such as breast, bladder, renal cell, ovarian, and lung cancers, where its downregulation or loss promotes tumor growth, stemness, epithelial-mesenchymal transition (EMT), and metastasis.

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