Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, U-251 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human heart tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
Product Information
27344-1-AP targets RARS in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26390 Product name: Recombinant human RARS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-190 aa of BC000528 Sequence: MDVLVSECSARLLQQEEEIKSLTAEIDRLKNCGCLGASPNLEQLQEENLKLKYRLNILRKSLQAERNKPTKNMINIISRLQEVFGHAIKAAYPDLENPPLLVTPSQQAKFGDYQCNSAMGISQMLKTKEQKVNPREIAENITKHLPDNECIEKVEIAGPGFINVHLRKDFVSEQLTSLLVNGVQLPALGE 相同性解析による交差性が予測される生物種 |
| Full Name | arginyl-tRNA synthetase |
| Calculated molecular weight | 75 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC000528 |
| Gene Symbol | RARS |
| Gene ID (NCBI) | 5917 |
| RRID | AB_2880849 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54136 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RARS antibody 27344-1-AP | Download protocol |
| IP protocol for RARS antibody 27344-1-AP | Download protocol |
| WB protocol for RARS antibody 27344-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants
| ||
Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation | ||
bioRxiv Arginine limitation causes a directed DNA sequence evolution response in colorectal cancer cells
| ||
Sci Adv Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells
| ||
Mol Neurobiol Verification of Pain-Related Neuromodulation Mechanisms of Calcitonin in Knee Osteoarthritis |
