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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using RARB Monoclonal antibody (60119-1-Ig)

    WB analysis of MCF-7 using 60119-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 60119-1-Ig (RARB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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RARB Monoclonal antibody

RARB Monoclonal Antibody for WB, ELISA
Cat No. 60119-1-Ig
Clone No.3G9F11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inMCF7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60119-1-Ig targets RARB in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RARB fusion protein Ag6013 相同性解析による交差性が予測される生物種
Full Name retinoic acid receptor, beta
Calculated molecular weight 50 kDa
GenBank accession numberBC060794
Gene Symbol RARB
Gene ID (NCBI) 5915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10826
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Retinoic acid receptors (RARs) are important mediators of retinoid signaling in morphogenesis, development, and cell differentiation. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes [PMID:12554770]. They can bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, they act mainly as activators of gene expression due to weak binding to corepressors. In concert with RARG, RARs are required for skeletal growth, matrix homeostasis and growth plate function. RARB is one isotypes of RARs [PMID:12834868]

Protocols

Product Specific Protocols
WB protocol for RARB antibody 60119-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols