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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAPGEF2 Polyclonal antibody (31299-1-AP)

    WB analysis using 31299-1-AP (same clone as 31299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31299-1-AP (RAPGEF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31299-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using RAPGEF2 Polyclonal antibody (31299-1-AP)

    IF Staining of mouse kidney using 31299-1-AP (same clone as 31299-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using RAPGEF2 antibody (31299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31299-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31299-1-PBS targets RAPGEF2 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAPGEF2 fusion protein Ag35288 相同性解析による交差性が予測される生物種
Full Name Rap guanine nucleotide exchange factor (GEF) 2
Calculated molecular weight167kDa
Observed molecular weight200 kDa
GenBank accession numberNM_014247.2
Gene Symbol RAPGEF2
Gene ID (NCBI) 9693
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9Y4G8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.