Validation Data Gallery
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Tested Applications
| Positive WB detected in | HCT 116 cells, HeLa cells, MCF-7 cells, SW480 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33845-1-AP targets RAP2C in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38723 Product name: Recombinant human RAP2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-183 aa of BC003403 Sequence: MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIVRVKRYEKVPLILVGNKVDLEPEREVMSSEGRALVQEWGCPFMETSAKSKSMVDELFAEIVRQMNYSSLPEKQDQCCTTCVVQ 相同性解析による交差性が予測される生物種 |
| Full Name | RAP2C, member of RAS oncogene family |
| Calculated molecular weight | 20 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC003403 |
| Gene Symbol | RAP2C |
| Gene ID (NCBI) | 57826 |
| RRID | AB_3743092 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y3L5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAP2C is a member of the Ras superfamily, which is the promoter of tumorigenesis. RAP2C plays a key role in the migration and invasion of various tumors, such as osteosarcoma, glioma, and small cell lung cancer (SCLC).(PMID: 29552178; PMID: 32341653; PMID: 33302819)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAP2C antibody 33845-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |