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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using RAP2A Polyclonal antibody (13789-1-AP)

    WB analysis of A431 using 13789-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RAP2A Polyclonal antibody (13789-1-AP)

    WB analysis of K-562 using 13789-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RAP2A Polyclonal antibody (13789-1-AP)

    WB analysis of PC-3 using 13789-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAP2A Polyclonal antibody (13789-1-AP)

    WB analysis of HeLa using 13789-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using RAP2A Polyclonal antibody (13789-1-AP)

    WB analysis of mouse lung using 13789-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RAP2A Polyclonal antibody (13789-1-AP)

    WB analysis of mouse brain using 13789-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAP2A Polyclonal antibody (13789-1-AP)

    WB analysis of HeLa using 13789-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RAP2A Polyclonal antibody (13789-1-AP)

    IF Staining of MCF-7 using 13789-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 13789-1-AP (RAP2A antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

RAP2A Polyclonal antibody

RAP2A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13789-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RbBP 30, RbBP-30, Ras-related protein Rap-2a, Ras related protein Rap 2a, RAP2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, mouse lung tissue, K-562 cells, mouse brain tissue, PC-3 cells, HeLa cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13789-1-AP targets RAP2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4808

Product name: Recombinant human RAP2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-183 aa of BC041333

Sequence: MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYEKVPVILVGNKVDLESEREVSSSEGRALAEEWGCPFMETSAKSKTMVDELFAEIVRQMNYAAQPDKDDPCCSACNIQ

相同性解析による交差性が予測される生物種
Full Name RAP2A, member of RAS oncogene family
Calculated molecular weight 183 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC041333
Gene Symbol RAP2A
Gene ID (NCBI) 5911
RRIDAB_2177303
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP10114
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for RAP2A antibody 13789-1-APDownload protocol
WB protocol for RAP2A antibody 13789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

miR-9 and miR-124 synergistically affect regulation of dendritic branching via the AKT/GSK3β pathway by targeting Rap2a.

Authors - Qian Xue
View Article
humanIHC, WB

Clin Exp Med

RAP2A promotes apoptosis resistance of hepatocellular carcinoma cells via the mTOR pathway.

Authors - Jing-Ru Yang
View Article
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