Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, Jurkat cells, K-562 cells, MOLT-4 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60940-1-Ig targets RAP1GDS1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32728 Product name: Recombinant human RAP1GDS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 176-406 aa of BC001851 Sequence: QLINMGVIPTLVKLLGIHCQNAALTEMCLVAFGNLAELESSKEQFASTNIAEELVKLFKKQIEHDKREMIFEVLAPLAENDAIKLQLVEAGLVECLLEIVQQKVDSDKEDDITELKTGSDLMVLLLLGDESMQKLFEGGKGSVFQRVLSWIPSNNHQLQLAGALAIANFARNDANCIHMVDNGIVEKLMDLLDRHVEDGNVTVQHAALSALRNLAIPVINKAKMLSAGVTE 相同性解析による交差性が予測される生物種 |
| Full Name | RAP1, GTP-GDP dissociation stimulator 1 |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 55-66 kDa |
| GenBank accession number | BC001851 |
| Gene Symbol | RAP1GDS1 |
| Gene ID (NCBI) | 5910 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P52306 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GDS stimulates the GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins), including at least c-Ki-ras p21, smg p21A, smg p21B, rhoA p21 and rhoB p21, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. The small GTPase Rap1, which is activated by a large variety of stimuli, functions in the control of integrin-mediated cell adhesion. RAP1GDS1 (synonym: GDS1)is one of RAP1 GTP-GDP dissociation stimulators.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAP1GDS1 antibody 60940-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |