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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAP1A Recombinant monoclonal antibody (83708-1-RR)

    WB analysis using 83708-1-RR (same clone as 83708-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83708-1-RR (RAP1A antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using RAP1A Recombinant monoclonal antibody (83708-1-RR)

    WB analysis of A431 using 83708-1-RR (same clone as 83708-1-PBS)

    WB result of RAP1A antibody (83708-1-RR; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAP1A transfected A431 cells. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RAP1A Recombinant monoclonal antibody (83708-1-RR)

    IF Staining of NIH/3T3 using 83708-1-RR (same clone as 83708-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAP1A antibody (83708-1-RR, Clone: 240438G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RAP1A Recombinant monoclonal antibody (83708-1-RR)

    FC experiment of HeLa using 83708-1-RR (same clone as 83708-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-1-RR, Clone:240438G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83708-1-RR

    Affinity and Kinetic Characterization of 83708-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83708-1-RR against Human RAP1A were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

RAP1A Recombinant monoclonal antibody, PBS Only

RAP1A Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83708-1-PBS
Clone No.240438G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240438G5, EC:3.6.5.2, G-22K, GTP-binding protein smg p21A, KREV1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83708-1-PBS targets RAP1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9488

Product name: Recombinant human RAP1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-184 aa of BC014086

Sequence: MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL

相同性解析による交差性が予測される生物種
Full Name RAP1A, member of RAS oncogene family
Calculated molecular weight 184 aa, 21 kDa
GenBank accession numberBC014086
Gene Symbol RAP1A
Gene ID (NCBI) 5906
RRIDAB_3671310
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP62834
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).

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