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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAP1A Recombinant antibody (83708-1-RR)

    WB analysis using 83708-1-RR (same clone as 83708-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83708-1-RR (RAP1A antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using RAP1A Recombinant antibody (83708-1-RR)

    WB analysis of A431 using 83708-1-RR (same clone as 83708-1-PBS)

    WB result of RAP1A antibody (83708-1-RR; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAP1A transfected A431 cells. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RAP1A Recombinant antibody (83708-1-RR)

    IF Staining of NIH/3T3 using 83708-1-RR (same clone as 83708-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAP1A antibody (83708-1-RR, Clone: 240438G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RAP1A Recombinant antibody (83708-1-RR)

    FC experiment of HeLa using 83708-1-RR (same clone as 83708-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-1-RR, Clone:240438G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83708-1-RR

    Affinity and Kinetic Characterization of 83708-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83708-1-RR against Human RAP1A were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

RAP1A Recombinant antibody, PBS Only

RAP1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83708-1-PBS
Clone No.240438G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240438G5, EC:3.6.5.2, G-22K, GTP-binding protein smg p21A, KREV1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83708-1-PBS targets RAP1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAP1A fusion protein Ag9488 相同性解析による交差性が予測される生物種
Full Name RAP1A, member of RAS oncogene family
Calculated molecular weight 184 aa, 21 kDa
GenBank accession numberBC014086
Gene Symbol RAP1A
Gene ID (NCBI) 5906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62834
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).