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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RANBP3L Polyclonal antibody (17875-1-AP)

    WB analysis using 17875-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17875-1-AP (RANBP3L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RANBP3L Polyclonal antibody (17875-1-AP)

    WB analysis of Jurkat using 17875-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17875-1-AP (RANBP3L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RANBP3L Polyclonal antibody (17875-1-AP)

    WB analysis of Jurkat using 17875-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17875-1-AP (RANBP3L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RANBP3L Polyclonal antibody (17875-1-AP)

    IHC staining of mouse kidney using 17875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RANBP3L Polyclonal antibody (17875-1-AP)

    IHC staining of mouse kidney using 17875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RANBP3L Polyclonal antibody (17875-1-AP)

    IHC staining of mouse brain using 17875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RANBP3L Polyclonal antibody (17875-1-AP)

    IHC staining of mouse brain using 17875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RANBP3L Polyclonal antibody

RANBP3L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17875-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

RAN binding protein 3 like, RANBP3L

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inmouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17875-1-AP targets RANBP3L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11642

Product name: Recombinant human RANBP3L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 110-465 aa of BC047660

Sequence: SAEQGPVKHSKHVIRPAILQLPQARSCAKVRKTFGHKALESCKTKEKTNNKISEGNSYLLSENLSRARISVQLSTNQDFLGATSVGCQPNEDKCSFKSCSSNFVFGENMVERVLGTQKLTQPQLENDSYAKEKPFKSIPKFPVNFLSSRTDSIKNTSLIESAAAFSSQPSRKCLLEKIDVITGEETEHNVLKINCKLFIFNKTTQSWIERGRGTLRLNDTASTDCGTLQSRLIMRNQGSLRLILNSKLWAQMKIQRANHKNVRITATDLEDYSIKIFLIQASAQDTAYLYAAIHHRLVALQSFNKQRDVNQAESLSETAQQLNCESCDENEDDFIQVTKNGSDPSSWTHRQSVACS

相同性解析による交差性が予測される生物種
Full Name RAN binding protein 3-like
Calculated molecular weight 465 aa, 52 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC047660
Gene Symbol RANBP3L
Gene ID (NCBI) 202151
RRIDAB_2284894
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for RANBP3L antibody 17875-1-APDownload protocol
WB protocol for RANBP3L antibody 17875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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