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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

RANBP10 Polyclonal antibody

RANBP10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21107-1-AP

Synonyms

KIAA1464, RAN binding protein 10, RANBP10

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 21107-1-AP

    WB analysis of MCF-7 using 21107-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 21107-1-AP

    WB analysis of mouse brain using 21107-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 21107-1-AP

    WB analysis of mouse heart using 21107-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 21107-1-AP

    WB analysis of mouse skeletal muscle using 21107-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 21107-1-AP

    WB analysis of LNCaP using 21107-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 21107-1-AP

    IHC staining of human hepatocirrhosis using 21107-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with RANBP10 antibody included
  • IHC staining of human hepatocirrhosis using 21107-1-AP

    IHC staining of human hepatocirrhosis using 21107-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with RANBP10 antibody included
  • IHC staining of human heart using 21107-1-AP

    IHC staining of human heart using 21107-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with RANBP10 antibody included
  • IHC staining of human heart using 21107-1-AP

    IHC staining of human heart using 21107-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with RANBP10 antibody included

Tested Applications

Positive WB detected inMCF-7 cells, mouse skeletal muscle tissue, mouse heart tissue, LNCaP cells, mouse brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RANBP10 Ready-To-Use IHC Kit. RANBP10 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21107-1-AP is RANBP10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RANBP10 fusion protein Ag15387 相同性解析による交差性が予測される生物種
Full Name RAN binding protein 10
Calculated molecular weight 620 aa, 67 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC099917
Gene symbol RANBP10
Gene ID (NCBI) 57610
RRIDAB_10732603
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RANBP10 antibody 21107-1-APDownload protocol
IHC protocol for RANBP10 antibody 21107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Theranostics

miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease.

Authors - Lu-Shiun Her
View Article
humanWB

PLoS Pathog

A Ran-binding protein facilitates nuclear import of human papillomavirus type 16.

Authors - Kun-Yi Lai
  • KD Validated
View Article