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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAN Monoclonal antibody (67500-1-Ig)

    WB analysis using 67500-1-Ig (same clone as 67500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67500-1-Ig (RAN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RAN Monoclonal antibody (67500-1-Ig)

    WB analysis of HEK-293 using 67500-1-Ig (same clone as 67500-1-PBS)

    WB result of RAN antibody (67500-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells. This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAN Monoclonal antibody (67500-1-Ig)

    IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAN Monoclonal antibody (67500-1-Ig)

    IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAN Monoclonal antibody (67500-1-Ig)

    IHC staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAN Monoclonal antibody (67500-1-Ig)

    IHC staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using RAN Monoclonal antibody (67500-1-Ig)

    IF Staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RAN antibody (67500-1-Ig, Clone: 1B11E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RAN Monoclonal antibody, PBS Only

RAN Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67500-1-PBS
Clone No.1B11E1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

ARA24, 1B11E1, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67500-1-PBS targets RAN in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30034

Product name: Recombinant human RAN protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-216 aa of BC004272

Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL

相同性解析による交差性が予測される生物種
Full Name RAN, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC004272
Gene Symbol RAN
Gene ID (NCBI) 5901
RRIDAB_2882724
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP62826
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).

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