Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10469-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10469-1-AP
WB result of RAN antibody (10469-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells.
WB analysis of A375 using 10469-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10469-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10469-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 10469-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10469-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10469-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 10469-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 10469-1-AP
IP result of anti-RAN (IP:10469-1-AP, 4ug; Detection:10469-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.
IHC staining of human breast cancer using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded normal colon slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10469-1-AP (RAN antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10469-1-AP (RAN antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10469-1-AP (RAN Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10469-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10469-1-AP (RAN Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 10469-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAN antibody (10469-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
FC experiment of Jurkat using 10469-1-AP
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RAN Polyclonal antibody (10469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
FC experiment of HeLa using 10469-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAN Polyclonal antibody (10469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

RAN Polyclonal antibody

RAN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10469-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ARA24, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran, GTPase Ran

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A375 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse kidney tissue, NIH/3T3 cells, rat kidney tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human breast cancer tissue, human colon tissue, human placenta tissue, human testis tissue, mouse colon tissue, mouse testis tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells
Positive FC (Intra) detected in	HeLa cells, Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 13 publications below
IHC	See 3 publications below
IF	See 6 publications below
IP	See 3 publications below

Product Information

10469-1-AP targets RAN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken, macrobrachium rosenbergii
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0748 Product name: Recombinant human RAN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC004272 Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL 相同性解析による交差性が予測される生物種
Full Name	RAN, member RAS oncogene family
Calculated molecular weight	25 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC004272
Gene Symbol	RAN
Gene ID (NCBI)	5901
RRID	AB_2176484
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62826
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for RAN antibody 10469-1-AP	Download protocol
IHC protocol for RAN antibody 10469-1-AP	Download protocol
IP protocol for RAN antibody 10469-1-AP	Download protocol
WB protocol for RAN antibody 10469-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP,IF	Cell Death Dis RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression. Authors - Xunhua Liu KD Validated View Article
rat,human	WB	Diabetologia Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function. Authors - Mu Zhang View Article
rat	WB,IP	Cell Commun Signal Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression. Authors - Abiola Abdulrahman Ayanlaja View Article
human	IHC	Oncotarget Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS. Authors - Zhen Chen View Article
human	WB	Int J Biol Sci Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression. Authors - Yancheng Zhong KD Validated View Article
human	IF	J Cell Sci A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex. Authors - Mohamed Hamed View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10469-1-AP

WB analysis of HEK-293 using 10469-1-AP

WB analysis of A375 using 10469-1-AP

WB analysis of HeLa using 10469-1-AP

WB analysis of Jurkat using 10469-1-AP

WB analysis of rat kidney using 10469-1-AP

WB analysis of NIH/3T3 using 10469-1-AP

WB analysis of mouse kidney using 10469-1-AP

WB analysis of A375 using 10469-1-AP

IP experiment of mouse kidney using 10469-1-AP

IHC staining of human breast cancer using 10469-1-AP

IHC staining of human breast cancer using 10469-1-AP

IHC staining of mouse colon using 10469-1-AP

IHC staining of mouse testis using 10469-1-AP

IHC staining of rat colon using 10469-1-AP

IHC staining of human placenta using 10469-1-AP

IHC staining of human colon using 10469-1-AP

IHC staining of human testis using 10469-1-AP

IHC staining of human testis using 10469-1-AP

IHC staining of human testis using 10469-1-AP

IHC staining of human testis using 10469-1-AP

IF Staining of HEK-293 using 10469-1-AP

FC experiment of Jurkat using 10469-1-AP

FC experiment of HeLa using 10469-1-AP

RAN Polyclonal antibody

RAN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS.

Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.

A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex.