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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAMP2 Recombinant monoclonal antibody (88020-3-RR)

    WB analysis using 88020-3-RR (same clone as 88020-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88020-3-RR (RAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88020-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using RAMP2 Recombinant monoclonal antibody (88020-3-RR)

    WB analysis of mouse lung using 88020-3-RR (same clone as 88020-3-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 88020-3-RR (RAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88020-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lung tissue using RAMP2 Recombinant monoclonal antibody (88020-3-RR)

    WB analysis of rat lung using 88020-3-RR (same clone as 88020-3-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 88020-3-RR (RAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88020-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using RAMP2 Recombinant monoclonal antibody (88020-3-RR)

    IHC staining of mouse lung using 88020-3-RR (same clone as 88020-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 88020-3-RR (RAMP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88020-3-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03316-1

    Cytometric bead array sample test of MP03316-1

    The mean RAMP2 concentration was determined to be 2.1 ng/mL in mouse brain tissue extract based on a 1.0 mg/mL extract load, 408.1 ng/mL in mouse lung tissue extract based on a 1.0 mg/mL extract load, 1.1 ng/mL in mouse retina tissue extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP03316-1

    Cytometric bead array standard curve of MP03316-1

    Cytometric bead array standard curve of MP03316-1, MOUSE RAMP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88020-1-PBS. Detection antibody: 88020-3-PBS. Standard: Eg9065. Range: 0.156-20 ng/mL.

Mouse RAMP2 Recombinant monoclonal antibody, PBS Only (Detector)

RAMP2 Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 88020-3-PBS
Clone No.260084E10

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

Receptor activity-modifying protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88020-3-PBS targets RAMP2 as part of a matched antibody pair:

MP03316-1: 88020-1-PBS capture and 88020-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg9065

Product name: Recombinant Mouse RAMP2 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 45-157 aa of NM_019444.2

Sequence: SPESLNQSLPESQNQSHPTEDSLVSKGKMEDYETHVLPCWYEYKSCMDSVKDWCNWTLISRHYSDLQNCLEYNADKFGLGFPNPLAENIILEAHLIHFANCSLVQPTFSDPPE

相同性解析による交差性が予測される生物種
Full Name receptor (calcitonin) activity modifying protein 2
Calculated molecular weight21 kDa
Observed molecular weight20-35kDa
GenBank accession numberNM_019444.2
Gene Symbol Ramp2
Gene ID (NCBI) 54409
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9WUP0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAMP2 (receptor-activity-modifying protein) is a member of the RAMP family of single-transmembrane-domain proteins which consist of an N-terminal extracellular domain, a transmembrane region and a short intracellular C-terminal tail. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a G protein-coupled receptor, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. RAMP1 transports the CRLR to the plasma membrane and then remains associated with it to function as a terminally glycosylated CGRP receptor, while RAMP2 and RAMP3 transfer the CRLR to the cell surface to generate receptors that are preferentially selective for adrenomedullin. (PMID: 9620797; 16188935)

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