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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RALYL Polyclonal antibody (28706-1-AP)

    WB analysis using 28706-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28706-1-AP (RALYL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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RALYL Polyclonal antibody

RALYL Polyclonal Antibody for WB, ELISA
Cat No. 28706-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

hnRNP core protein C like 3, HNRPCL3, hRALYL, RALY RNA binding protein like, RALYL, RNA binding Raly like protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28706-1-AP targets RALYL in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RALYL fusion protein Ag30310 相同性解析による交差性が予測される生物種
Full Name RALY RNA binding protein-like
Calculated molecular weight 291 aa, 32 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC031090
Gene Symbol RALYL
Gene ID (NCBI) 138046
RRIDAB_2881197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86SE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RALY RNA binding protein-like (RALYL) belongs to the RALY subfamily. RALYL locates in 8q21.2, the function of which is RNA-binding and nucleotide binding. The RALYL high expression in the normal adrenal, kidney and brain (Shyamsundar et al., 2005). Furthermore, RALYL expression down-regulated correlates with mental disorder (Lee et al., 2007), Parkinson's disease (Moran et al., 2006), brain cancer (Maris et al., 2008), adrenal cancer (Giordano et al., 2009) and kidney cancer (Higgins et al., 2003). The expression of RALYL was significantly low in Clear Cell Renal Cell Carcinoma and correlated with a poor prognosis in a large number of clinical samples. Our findings showed that RALYL may be a potential therapeutic target as well as a poor prognostic

Protocols

Product Specific Protocols
WB protocol for RALYL antibody 28706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols