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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RALBP1 Polyclonal antibody

RALBP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13176-1-AP

Synonyms

76 kDa Ral interacting protein, DNP SG ATPase, Ral interacting protein 1, ralA binding protein 1, RALBP1, RIP1, RLIP1, RLIP76

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 13176-1-AP

    WB analysis of K-562 using 13176-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13176-1-AP (RALBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 13176-1-AP

    WB analysis of HEK-293 using 13176-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13176-1-AP (RALBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 13176-1-AP

    WB analysis of HepG2 using 13176-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13176-1-AP (RALBP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 13176-1-AP

    WB analysis of MCF-7 using 13176-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13176-1-AP (RALBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 13176-1-AP

    IP experiment of K-562 using 13176-1-AP

    IP result of anti-RALBP1 (IP:13176-1-AP, 4ug; Detection:13176-1-AP 1:800) with K-562 cells lysate 3600ug.

Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, HepG2 cells, MCF-7 cells
Positive IP detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy RALBP1 Ready-To-Use IHC Kit. RALBP1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

13176-1-AP targets RALBP1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RALBP1 fusion protein Ag3843 相同性解析による交差性が予測される生物種
Full Name ralA binding protein 1
Calculated molecular weight 655 aa, 76 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC013126
Gene symbol RALBP1
Gene ID (NCBI) 10928
RRIDAB_2176171
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RALBP1 antibody 13176-1-APDownload protocol
IP protocol for RALBP1 antibody 13176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Neoplasia

PMID: 28092822

Authors - Jian Yang
View Article