• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RALB Polyclonal antibody

RALB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12340-1-AP

Synonyms

RALB, Ras related protein Ral B

Validation Data Gallery

Applications:
  • WB analysis of Y79 using 12340-1-AP

    WB analysis of Y79 using 12340-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 12340-1-AP

    WB analysis of human brain using 12340-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human lung using 12340-1-AP

    WB analysis of human lung using 12340-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Y79 using 12340-1-AP

    WB analysis of Y79 using 12340-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 12340-1-AP

    WB analysis of mouse lung using 12340-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 12340-1-AP

    WB analysis of mouse brain using 12340-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 12340-1-AP

    IHC staining of human ovary tumor using 12340-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of human ovary tumor using 12340-1-AP

    IHC staining of human ovary tumor using 12340-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of human colon using 12340-1-AP

    IHC staining of human colon using 12340-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of human colon using 12340-1-AP

    IHC staining of human colon using 12340-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with RALB antibody included

Tested Applications

Positive WB detected inY79 cells, human brain tissue, human lung tissue, mouse brain tissue, mouse lung tissue
Positive IHC detected inhuman ovary tumor tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RALB Ready-To-Use IHC Kit. RALB primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12340-1-AP targets RALB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity Drosophila, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RALB fusion protein Ag2992 相同性解析による交差性が予測される生物種
Full Name v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Calculated molecular weight 206 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC018163
Gene symbol RALB
Gene ID (NCBI) 5899
RRIDAB_2269253
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RALB antibody 12340-1-APDownload protocol
IHC protocol for RALB antibody 12340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
DrosophilaIHC

Development

Ral GTPase promotes asymmetric Notch activation in the Drosophila eye in response to Frizzled/PCP signaling by repressing ligand-independent receptor activation.

Authors - Cho Bomsoo B
View Article
humanWB

Front Chem

Design, Synthesis, and Biological Evaluation of Pyrano[2,3-c]-pyrazole-Based RalA Inhibitors Against Hepatocellular Carcinoma.

Authors - Yuting Wang
View Article
humanWB,IHC

Arch Biochem Biophys

MicroRNA-139 inhibits pancreatic-cancer carcinogenesis by suppressing RalB via the Ral/RAC/PI3K pathway.

Authors - Yan Wang
View Article