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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAI2 Polyclonal antibody (28217-1-AP)

    WB analysis using 28217-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28217-1-AP (RAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SW480 cells using RAI2 Polyclonal antibody (28217-1-AP)

    IF Staining of SW480 using 28217-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using RAI2 antibody (28217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RAI2 Polyclonal antibody

RAI2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28217-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

retinoic acid induced 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, IMR-32 cells, SK-N-SH cells, TT cells
Positive IF/ICC detected inSW480 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28217-1-AP targets RAI2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAI2 fusion protein Ag28253 相同性解析による交差性が予測される生物種
Full Name retinoic acid induced 2
Calculated molecular weight 57 kDa
Observed molecular weight70 kDa
GenBank accession numberBC027937
Gene Symbol RAI2
Gene ID (NCBI) 10742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5P3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Retinoic acid-induced 2 (RAI2) was initially described as a retinoic acid-inducible gene, which has been assumed to be involved in development. RAI2 was initially related to cell differentiation and induced by retinoic acid and has been identified as an emerging tumor suppressor in breast cancer and colorectal cancer. RAI2 is frequently methylated in human CRC, and the expression of RAI2 is regulated by promoter region methylation (PMID: 29796120).

Protocols

Product Specific Protocols
WB protocol for RAI2 antibody 28217-1-APDownload protocol
IF protocol for RAI2 antibody 28217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols