Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
17084-1-AP targets RAI1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9046 Product name: Recombinant human RAI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC021209 Sequence: MQSFRERCGFHGKQQNYQQTSQETSRLENYRQPSQAGLSCDRQRLLAKDYYNPQPYPSYEGGAGTPSGTAAAVAADKYHRGSKALPTQQGLQGRPAFPGYGVQDSSPYPGRYAGEESLQAWGAPQPPPPQPQPLPAGVAKYDENLMKKTAVPPSRQYAEQGAQVPFRTHSLHVQQPPPPQQPLAYPKLQRQKLQNDIASPLPFPQGTHFPQHSQSFPTSSTYSSSVQGGGQGAHSYKSCTAPTAQPHDRPLTASSSLAPGQRVQNLHAYQSGRLSYDQQQQQQQQQQQQQQALQSRHHAQETLHYQNLAKYQHYGQQGQGYCQPDAAVRTPEQYYQTFSPSSSHSPARSV 相同性解析による交差性が予測される生物種 |
| Full Name | retinoic acid induced 1 |
| Calculated molecular weight | 1906aa,203 kDa; 966aa,104 kDa |
| Observed molecular weight | 250-260 kDa |
| GenBank accession number | BC021209 |
| Gene Symbol | RAI1 |
| Gene ID (NCBI) | 10743 |
| RRID | AB_2284824 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z5J4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The RAI1 (Retinoic Acid Induced 1) gene is located on the short arm of chromosome 17. The RAI1 protein it encodes is a crucial transcriptional regulator, playing a central role in processes such as neurodevelopment, sleep regulation, circadian rhythms, and energy metabolism. This protein contains a chromatin domain that binds to specific DNA sequences and recruits other transcriptional co-factors to activate or repress the expression of downstream target genes. The RAI1 gene is extremely sensitive to haploinsufficiency, and its dysfunction is closely linked to several severe neurodevelopmental disorders. For instance, RAI1 haploinsufficiency causes Smith-Magenis syndrome, while duplication of the RAI1 gene is associated with Potocki-Lupski syndrome. Consequently, the RAI1 protein is a key molecule for understanding brain function and behavioral regulation, and a major focus of research for related genetic syndromes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAI1 antibody 17084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |