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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAGE Polyclonal antibody (31122-1-AP)

    WB analysis using 31122-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31122-1-AP (RAGE antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using RAGE Polyclonal antibody (31122-1-AP)

    IHC staining of mouse lung using 31122-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31122-1-AP (RAGE antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RAGE Polyclonal antibody

RAGE Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31122-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Ager, Advanced glycosylation end product-specific receptor, MOK, RAGE 1, RAGE1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31122-1-AP targets RAGE in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAGE fusion protein Eg0873 相同性解析による交差性が予測される生物種
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight43 kDa
Observed molecular weight42-55 kDa
GenBank accession numberNM_007425.3
Gene Symbol Ager
Gene ID (NCBI) 11596
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ62151-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614).

Protocols

Product Specific Protocols
WB protocol for RAGE antibody 31122-1-APDownload protocol
IHC protocol for RAGE antibody 31122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols