Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of HeLa using 23926-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 23926-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23926-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human hepatocirrhosis using 23926-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).
IHC staining of human hepatocirrhosis using 23926-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 23926-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 23926-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 23926-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23926-1-AP (MOK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 23926-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug MOK Polyclonal antibody (23926-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

MOK Polyclonal antibody

MOK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 23926-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

RAGE, EC:2.7.11.22, MOK protein kinase, RAGE 1, RAGE1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells
Positive IHC detected in	human hepatocirrhosis tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below

Product Information

23926-1-AP targets MOK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20849 Product name: Recombinant human RAGE protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-231 aa of BC053536 Sequence: MKNYKAIGKIGEGTFSEVMKMQSLRDGNYYACKQMKQRFESIEQVNNLREIQALRRLNPHPNILMLHEVVFDRKSGSLALICELMDMNIYELIRGRRYPLSEKKIMHYMYQLCKSLDHIHRNGIFHRDVKPENILIKQDVLKLGDFGSCRSVYSKQPYTEYISTRWYRAPECLLTDGFYTYKMDLWSAGCVFYEIASLQPLFPGVNELDQISKIHDVIGTPAQKILTKFKQ 相同性解析による交差性が予測される生物種
Full Name	renal tumor antigen
Calculated molecular weight	231 aa, 27 kDa
Observed molecular weight	42 kDa, 85 kDa
GenBank accession number	BC053536
Gene Symbol	RAGE
Gene ID (NCBI)	5891
RRID	AB_2879364
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UQ07
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
FC protocol for MOK antibody 23926-1-AP	Download protocol
IF protocol for MOK antibody 23926-1-AP	Download protocol
IHC protocol for MOK antibody 23926-1-AP	Download protocol
WB protocol for MOK antibody 23926-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Front Oncol Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities Authors - Jing Hang KD Validated View Article
	WB	J Biol Chem Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins. Authors - Ran Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 23926-1-AP

WB analysis of HepG2 using 23926-1-AP

WB analysis of HeLa using 23926-1-AP

IHC staining of human hepatocirrhosis using 23926-1-AP

IHC staining of human hepatocirrhosis using 23926-1-AP

IHC staining of human heart using 23926-1-AP