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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MOK Polyclonal antibody (23926-1-AP)

    WB analysis of HeLa using 23926-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MOK Polyclonal antibody (23926-1-AP)

    WB analysis of HepG2 using 23926-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MOK Polyclonal antibody (23926-1-AP)

    WB analysis of HeLa using 23926-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using MOK Polyclonal antibody (23926-1-AP)

    IHC staining of human hepatocirrhosis using 23926-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using MOK Polyclonal antibody (23926-1-AP)

    IHC staining of human hepatocirrhosis using 23926-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MOK Polyclonal antibody (23926-1-AP)

    IHC staining of human heart using 23926-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MOK Polyclonal antibody (23926-1-AP)

    IHC staining of human heart using 23926-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MOK Polyclonal antibody (23926-1-AP)

    IF Staining of HeLa using 23926-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23926-1-AP (MOK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using MOK Polyclonal antibody (23926-1-AP)

    FC experiment of HeLa using 23926-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MOK Polyclonal antibody (23926-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

MOK Polyclonal antibody

MOK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 23926-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

RAGE, EC:2.7.11.22, MOK protein kinase, RAGE 1, RAGE1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23926-1-AP targets MOK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOK fusion protein Ag20849 相同性解析による交差性が予測される生物種
Full Name renal tumor antigen
Calculated molecular weight 231 aa, 27 kDa
Observed molecular weight 42 kDa, 85 kDa
GenBank accession numberBC053536
Gene Symbol RAGE
Gene ID (NCBI) 5891
RRIDAB_2879364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MOK antibody 23926-1-APDownload protocol
IHC protocol for MOK antibody 23926-1-APDownload protocol
IF protocol for MOK antibody 23926-1-APDownload protocol
FC protocol for MOK antibody 23926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities

Authors - Jing Hang
  • KD Validated
View Article
WB

J Biol Chem

Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins.

Authors - Ran Li
View Article