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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAE1 Recombinant antibody (83712-4-RR)

    WB analysis using 83712-4-RR (same clone as 83712-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-4-RR (RAE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RAE1 Recombinant antibody (83712-4-RR)

    IHC staining of mouse testis using 83712-4-RR (same clone as 83712-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83712-4-RR (RAE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAE1 Recombinant antibody (83712-4-RR)

    IF Staining of HepG2 using 83712-4-RR (same clone as 83712-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAE1 antibody (83712-4-RR, Clone: 240861B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83712-4-RR

    Affinity and Kinetic Characterization of 83712-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-4-RR against Human RAE1 were performed. The affinity constant is 20.7 nM.

RAE1 Recombinant monoclonal antibody, PBS Only

RAE1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83712-4-PBS
Clone No.240861B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83712-4-PBS targets RAE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag12102

Product name: Recombinant human RAE1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-368 aa of BC103754

Sequence: MSLFGTTSGFGTSGTSMFGSATTDNHNPMKDIEVTSSPDDSIGCLSFSPPTLPGNFLIAGSWANDVRCWEVQDSGQTIPKAQQMHTGPVLDVCWSDDGSKVFTASCDKTAKMWDLSSNQAIQIAQHDAPVKTIHWIKAPNYSCVMTGSWDKTLKFWDTRSSNPMMVLQLPERCYCADVIYPMAVVATAERGLIVYQLENQPSEFRRIESPLKHQHRCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNPQKKNYIFLRNAAEELKPRNKK

相同性解析による交差性が予測される生物種
Full Name RAE1 RNA export 1 homolog (S. pombe)
Calculated molecular weight 368 aa, 41 kDa
GenBank accession numberBC103754
Gene Symbol RAE1
Gene ID (NCBI) 8480
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP78406
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)

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