• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAD9A Polyclonal antibody

RAD9A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 13035-1-AP

Synonyms

hRAD9, RAD9, RAD9 homolog A (S. pombe), RAD9A

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 13035-1-AP

    WB analysis of HeLa using 13035-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 13035-1-AP

    WB analysis of MCF-7 using 13035-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 13035-1-AP

    WB analysis of A549 using 13035-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 13035-1-AP

    IHC staining of human breast cancer using 13035-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 13035-1-AP

    IHC staining of human breast cancer using 13035-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 13035-1-AP

    IF Staining of HeLa using 13035-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13035-1-AP (RAD9A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

13035-1-AP targets RAD9A in WB, IHC, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD9A fusion protein Ag3687 相同性解析による交差性が予測される生物種
Full Name RAD9 homolog A (S. pombe)
Calculated molecular weight 391 aa, 42 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC014848
Gene symbol RAD9A
Gene ID (NCBI) 5883
RRIDAB_10598474
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAD9A antibody 13035-1-APDownload protocol
IHC protocol for RAD9A antibody 13035-1-APDownload protocol
IF protocol for RAD9A antibody 13035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

Authors - Bin Chen
View Article
humanWB

J Mol Cell Biol

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Authors - Xiaoli Xu
View Article
humanWB

DNA Cell Biol

AEG-1 Knockdown Sensitizes Glioma Cells to Radiation Through Impairing Homologous Recombination Via Targeting RFC5.

Authors - Xu Zhao
View Article