Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, MDA-MB-231 cells, mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31678-1-AP targets RAD51C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35070 Product name: Recombinant human RAD51C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC107753 Sequence: MRGKTFRFEMQRDLVSFPLSPAVRVKLVSAGFQTAEELLEVKPSELSKEVGISKAEALETLQIIRRECLTNKPRYAGTSESHKKCTALELLEQEHTQGFIITFCSALDDILGGGVPLMKTTEICGAPGVGKTQLCMQLAVDVQIPECFGGVAGEAVFIDTEGSFMVDRVVDLATACIQHLQLIAEKHKGEEHRKALEDFTLDNILSHIYYFRCRDYTELLAQVYLLPDFLSEHSKVRLVIVDGIAFPFRHDLDDLSLRTRLLNGLAQQMISLANNHRLAVILTNQMTTKIDRNQALLVPALGESWGHAATIRLIFHWDRKQRLATLYKSPSQKECTVLFQIKPQGFRDTVVTSACSLQTEGSLSTRKRSRDPEEEL 相同性解析による交差性が予測される生物種 |
| Full Name | RAD51 homolog C (S. cerevisiae) |
| Calculated molecular weight | 376 aa, 42 kDa |
| Observed molecular weight | 40-42 kDa |
| GenBank accession number | BC107753 |
| Gene Symbol | RAD51C |
| Gene ID (NCBI) | 5889 |
| RRID | AB_3670072 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O43502 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RAD51C antibody 31678-1-AP | Download protocol |
| WB protocol for RAD51C antibody 31678-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |