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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD51AP1 Polyclonal antibody (11255-1-AP)

    WB analysis using 11255-1-AP (same clone as 11255-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using RAD51AP1 Polyclonal antibody (11255-1-AP)

    WB analysis of mouse liver using 11255-1-AP (same clone as 11255-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using RAD51AP1 Polyclonal antibody (11255-1-AP)

    WB analysis of rat liver using 11255-1-AP (same clone as 11255-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RAD51AP1 Polyclonal antibody (11255-1-AP)

    WB analysis of human brain using 11255-1-AP (same clone as 11255-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RAD51AP1 Polyclonal antibody (11255-1-AP)

    WB analysis of human brain using 11255-1-AP (same clone as 11255-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAD51AP1 Polyclonal antibody (11255-1-AP)

    IF Staining of HeLa using 11255-1-AP (same clone as 11255-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11255-1-AP (RAD51AP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RAD51AP1 Polyclonal antibody, PBS Only

RAD51AP1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 11255-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11255-1-PBS targets RAD51AP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1781

Product name: Recombinant human RAD51AP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-352 aa of BC016330

Sequence: MVRPVRHKKPVNYSQFDHSDSDDDFVSATVPLNKKSRTAPKELKQDKPKPNLNNLRKEEIPVQEKTPKKRLPEGTFSIPASAVPCTKMALDDKLYQRDLEVALALSVKELPTVTTNVQNSQDKSIEKHGSSKIETMNKSPHISNCSVASDYLDLDKITVEDDVGGVQGKRKAASKAAAQQRKILLEGSDGDSANDTEPDFAPGEDSEDDSDFCESEDNDEDFSMRKSKVKEIKKKEVKVKSPVEKKEKKSKSKCNALVTSVDSAPAAVKSESQSLPKKVSLSSDTTRKPLEIRSPSAESKKPKWVPPAASGGSRSSSSPLVVVSVKSPNQSLRLGLSRLARVKPLHPNATST

相同性解析による交差性が予測される生物種
Full Name RAD51 associated protein 1
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC016330
Gene Symbol RAD51AP1
Gene ID (NCBI) 10635
RRIDAB_2300786
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96B01
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].

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