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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD51 Recombinant monoclonal antibody (87990-1-RR)

    WB analysis using 87990-1-RR (same clone as 87990-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87990-1-RR (RAD51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87990-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAD51 Recombinant monoclonal antibody (87990-1-RR)

    WB analysis using 87990-1-RR (same clone as 87990-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87990-1-RR (RAD51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87990-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RAD51 Recombinant monoclonal antibody (87990-1-RR)

    IP experiment of HeLa using 87990-1-RR (same clone as 87990-1-PBS)

    IP result of anti-RAD51 (IP:87990-1-RR, 4ug; Detection:87990-1-RR 1:3000) with HeLa cells lysate 1000 ug. This data was developed using the same antibody clone with 87990-1-PBS in a different storage buffer formulation.

  • Affinity of 87990-1-RR

    Affinity of 87990-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87990-1-RR against Human RAD51 were performed. The affinity constant is below 1 pM.

RAD51 Recombinant monoclonal antibody, PBS Only

RAD51 Uni-rAb® Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 87990-1-PBS
Clone No.260095G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

BRCC5, HRAD51, HsRad51, HsT16930, RAD51 homolog A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87990-1-PBS targets RAD51 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6823

Product name: Recombinant human RAD51 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-242 aa of BC001459

Sequence: MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD

相同性解析による交差性が予測される生物種
Full Name RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC001459
Gene Symbol RAD51
Gene ID (NCBI) 5888
RRIDAB_3745846
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ06609
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

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