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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD50 Polyclonal antibody (29390-1-AP)

    WB analysis using 29390-1-AP (same clone as 29390-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29390-1-AP (RAD50 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using RAD50 Polyclonal antibody (29390-1-AP)

    WB analysis of MCF-7 using 29390-1-AP (same clone as 29390-1-PBS)

    WB result of RAD50 antibody (29390-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD50 transfected MCF-7 cells. This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of human placenta using 29390-1-AP (same clone as 29390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of human placenta using 29390-1-AP (same clone as 29390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IF Staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using RAD50 antibody (29390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAD50 Polyclonal antibody (29390-1-AP)

    IF Staining of HeLa using 29390-1-AP (same clone as 29390-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RAD50 Polyclonal antibody (29390-1-AP)

    IF Staining of U2OS using 29390-1-AP (same clone as 29390-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RAD50 Polyclonal antibody, PBS Only

RAD50 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 29390-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

hRad50, RAD50 homolog (S. cerevisiae)

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29390-1-PBS targets RAD50 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29949

Product name: Recombinant human RAD50 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 275-425 aa of NM_005732

Sequence: LDSRKKQMEKDNSELEEKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCHRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQEHIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEGEAKTANQLMNDFAEKETLK

相同性解析による交差性が予測される生物種
Full Name RAD50 homolog (S. cerevisiae)
Calculated molecular weight 154 kDa
Observed molecular weight 154 kDa
GenBank accession numberNM_005732
Gene Symbol RAD50
Gene ID (NCBI) 10111
RRIDAB_2918289
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92878
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).

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