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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD21 Recombinant monoclonal antibody (84920-4-RR)

    WB analysis using 84920-4-RR (same clone as 84920-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84920-4-RR (RAD21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84920-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RAD21 Recombinant monoclonal antibody (84920-4-RR)

    WB analysis of HeLa using 84920-4-RR (same clone as 84920-4-PBS)

    WB result of RAD21 antibody (84920-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD21 transfected HeLa cells. This data was developed using the same antibody clone with 84920-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAD21 Recombinant monoclonal antibody (84920-4-RR)

    IF Staining of HeLa using 84920-4-RR (same clone as 84920-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD21 antibody (84920-4-RR, Clone: 242172E3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84920-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using RAD21 Recombinant monoclonal antibody (84920-4-RR)

    FC experiment of Jurkat using 84920-4-RR (same clone as 84920-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RAD21 Recombinant antibody (84920-4-RR, Clone:242172E3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84920-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84920-4-RR

    Affinity and Kinetic Characterization of 84920-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84920-4-RR against Human RAD21 were performed. The affinity constant is 2.23 nM.

  • Featured Product
  • KD/KO Validated

RAD21 Recombinant monoclonal antibody, PBS Only

RAD21 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84920-4-PBS
Clone No.242172E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242172E3, 64-kDa C-terminal product, Double-strand-break repair protein rad21 homolog, FLJ25655, FLJ40596

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84920-4-PBS targets RAD21 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25481

Product name: Recombinant human RAD21 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 147-349 aa of BC050381

Sequence: EEVGNISILQENDFGDFGMDDREIMREGSAFEDDDMLVSTTTSNLLLESEQSTSNLNEKINHLEYEDQYKDDNFGEGNDGGILDDKLISNNDGGIFDDPPALSEAGVMLPEQPAHDDMDEDDNVSMGGPDSPDSVDPVEPMPTMTDQTTLVPNEEEAFALEPIDITVKETKAKRKRKLIVDSVKELDSKTIRAQLSDYSDIVT

相同性解析による交差性が予測される生物種
Full Name RAD21 homolog (S. pombe)
Calculated molecular weight 631 aa, 72 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC050381
Gene Symbol RAD21
Gene ID (NCBI) 5885
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO60216
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

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