Validation Data Gallery
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Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells, Daudi cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68539-1-Ig targets RAD17 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RAD17 fusion protein Ag4348 相同性解析による交差性が予測される生物種 |
| Full Name | RAD17 homolog (S. pombe) |
| Calculated molecular weight | 681 aa, 71 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC032304 |
| Gene Symbol | RAD17 |
| Gene ID (NCBI) | 5884 |
| RRID | AB_3085247 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O75943 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rad17, a component of the checkpoint clamp loader complex (RAD17/RFC), was originally initially identified as a DNA damage checkpoint protein. Rad17 participates in cell cycle control and DNA damage repair. Rad17 phosphorylation by ATR (ataxia telangiectasia mutated and Rad3-related) kinase is indispensable for the recruitment of Claspin and the activation of Chk1 in response to genotoxic stress.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAD17 antibody 68539-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |