Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30042-1-AP targets RACGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32468 Product name: Recombinant human RACGAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 540-632 aa of BC032754 Sequence: SQFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRSTLTKNTPRFGSKSKSATNLGRQGNFFASPMLK 相同性解析による交差性が予測される生物種 |
| Full Name | Rac GTPase activating protein 1 |
| Calculated molecular weight | 632 aa, 71 kDa |
| Observed molecular weight | 71 kDa |
| GenBank accession number | BC032754 |
| Gene Symbol | RACGAP1 |
| Gene ID (NCBI) | 29127 |
| RRID | AB_3086216 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0H5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RACGAP1 antibody 30042-1-AP | Download protocol |
| IHC protocol for RACGAP1 antibody 30042-1-AP | Download protocol |
| WB protocol for RACGAP1 antibody 30042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
