Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of K-562 using 13739-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 13739-1-AP
WB result of RACGAP1 antibody (13739-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RACGAP1 transfected A431 cells.
WB analysis of RAW 264.7 using 13739-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13739-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13739-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 13739-1-AP
IP result of anti-RACGAP1 (IP:13739-1-AP, 4ug; Detection:13739-1-AP 1:500) with mouse testis tissue lysate 4000ug.
IP experiment of K-562 using 13739-1-AP
IP and Co-IP result of anti-RACGAP1 (IP:13739-1-AP, 4ug); Detection antibodies: RACGAP1 (13739-1-AP 1:5000) and KIF23 (28587-1-AP 1:2000) with K-562 cells lysate 2440 ug.
IP experiment of HeLa cells using 13739-1-AP
IP result of anti-RACGAP1 (13739-1-AP for IP and Detection).
IF Staining of HepG2 using 13739-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RACGAP1 antibody (13739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

RACGAP1 Polyclonal antibody

RACGAP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 13739-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CYK4, GAP, Gap1, HsCYK 4, HsCYK4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, A431 cells, HeLa cells, mouse testis tissue, RAW 264.7 cells
Positive IP detected in	mouse testis tissue, HeLa cells, K-562 cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 10 publications below
IHC	See 10 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

13739-1-AP targets RACGAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4676 Product name: Recombinant human RACGAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 334-632 aa of BC032754 Sequence: PCIPTLIGTPVKIGEGMLADFVSQTSPMIPSIVVHCVNEIEQRGLTETGLYRISGCDRTVKELKEKFLRVKTVPLLSKVDDIHAICSLLKDFLRNLKEPLLTFRLNRAFMEAAEITDEDNSIAAMYQAVGELPQANRDTLAFLMIHLQRVAQSPHTKMDVANLAKVFGPTIVAHAVPNPDPVTMLQDIKRQPKVVERLLSLPLEYWSQFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRSTLTKNTPRFGSKSKSATNLGRQGNFFASPMLK 相同性解析による交差性が予測される生物種
Full Name	Rac GTPase activating protein 1
Calculated molecular weight	632 aa, 71 kDa
Observed molecular weight	71 kDa
GenBank accession number	BC032754
Gene Symbol	RACGAP1
Gene ID (NCBI)	29127
RRID	AB_2176276
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H0H5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

Protocols

Product Specific Protocols
IF protocol for RACGAP1 antibody 13739-1-AP	Download protocol
IP protocol for RACGAP1 antibody 13739-1-AP	Download protocol
WB protocol for RACGAP1 antibody 13739-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Death Dis Pseudogene RACGAP1P activates RACGAP1/Rho/ERK signalling axis as a competing endogenous RNA to promote hepatocellular carcinoma early recurrence. Authors - Meng-Yao Wang View Article
human	WB,IHC,IF	Cell Death Dis PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling Authors - Hualin Chen View Article
human	WB,IHC	Apoptosis Oncogenic and immunological roles of RACGAP1 in pan-cancer and its potential value in nasopharyngeal carcinoma Authors - Cheng-Shan Zheng View Article
human	IHC	Int Immunopharmacol RACGAP1 is a pivotal gene in lung adenocarcinoma-associated membranous nephropathy: Based on comprehensive bioinformatics analysis and machine learning Authors - Qianqian Xu View Article
mouse,human	WB,IHC,CoIP	Am J Physiol Cell Physiol Biological and metabolomic insights into RACGAP1-mediated growth and progression of clear cell renal cell carcinoma Authors - Wanyi Li KD Validated View Article
mouse	WB,IHC	Front Pharmacol Effects of Shenkang Pills on Early-Stage Diabetic Nephropathy in db/db Mice via Inhibiting AURKB/RacGAP1/RhoA Signaling Pathway. Authors - Fujing Wang View Article

Validation Data Gallery

WB analysis of K-562 using 13739-1-AP

WB analysis of A431 using 13739-1-AP

WB analysis of RAW 264.7 using 13739-1-AP

WB analysis of mouse testis using 13739-1-AP

WB analysis of HeLa using 13739-1-AP

IP experiment of mouse testis using 13739-1-AP

IP experiment of K-562 using 13739-1-AP

IP experiment of HeLa cells using 13739-1-AP

IF Staining of HepG2 using 13739-1-AP

RACGAP1 Polyclonal antibody

RACGAP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pseudogene RACGAP1P activates RACGAP1/Rho/ERK signalling axis as a competing endogenous RNA to promote hepatocellular carcinoma early recurrence.

PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling

Oncogenic and immunological roles of RACGAP1 in pan-cancer and its potential value in nasopharyngeal carcinoma

RACGAP1 is a pivotal gene in lung adenocarcinoma-associated membranous nephropathy: Based on comprehensive bioinformatics analysis and machine learning

Biological and metabolomic insights into RACGAP1-mediated growth and progression of clear cell renal cell carcinoma

Effects of Shenkang Pills on Early-Stage Diabetic Nephropathy in db/db Mice via Inhibiting AURKB/RacGAP1/RhoA Signaling Pathway.

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