Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of human lung using 11916-1-AP (same clone as 11916-1-PBS)
human lung tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
WB analysis using 11916-1-AP (same clone as 11916-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 11916-1-AP (same clone as 11916-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
WB analysis of HUVEC using 11916-1-PBS
HUVEC cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11916-1-AP (same clone as 11916-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 11916-1-AP (same clone as 11916-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IP experiment of mouse lung using 11916-1-AP (same clone as 11916-1-PBS)
IP result of anti-RABL3 (IP:11916-1-AP, 3ug; Detection:11916-1-AP 1:300) with mouse lung tissue lysate 4000ug. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 11916-1-AP (same clone as 11916-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RABL3 antibody (11916-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
FC experiment of HEK-293 using 11916-1-AP (same clone as 11916-1-PBS)
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human RABL3 (11916-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.
×
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human RABL3 (11916-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11916-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

RABL3 Polyclonal antibody, PBS Only

RABL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11916-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11916-1-PBS targets RABL3 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2531 Product name: Recombinant human RABL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-236 aa of BC020832 Sequence: MASLDRVKVLVLGDSGVGKSSLVHLLCQNQVLGNPSWTVGCSVDVRVHDYKEGTPEEKTCYIELWDVGGSVGSASSVKSTRAVFYNSVNGIIFVHDLTNKKSSQNLRRWSLEALNRDLVPTGVLVTNGDYDQEQFADNQIPLLVIGTKLDQIHETKRHEVLTTTAFLAEDFNPEEINLDCTNPRYLAAGSSNAVKLSRFFDKVIEKRYFLREGNQIPGFPDRKRFGAGTLKSLHYD 相同性解析による交差性が予測される生物種
Full Name	RAB, member of RAS oncogene family-like 3
Calculated molecular weight	236 aa, 26 kDa
Observed molecular weight	26 kDa
GenBank accession number	BC020832
Gene Symbol	RABL3
Gene ID (NCBI)	285282
RRID	AB_2175960
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5HYI8
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human lung using 11916-1-AP (same clone as 11916-1-PBS)

WB analysis using 11916-1-AP (same clone as 11916-1-PBS)

WB analysis of mouse brain using 11916-1-AP (same clone as 11916-1-PBS)

WB analysis of HUVEC using 11916-1-PBS

WB analysis of HEK-293 using 11916-1-AP (same clone as 11916-1-PBS)

WB analysis of human brain using 11916-1-AP (same clone as 11916-1-PBS)

IP experiment of mouse lung using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human liver cancer using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human liver cancer using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human breast cancer using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human cervical cancer using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human cervical cancer using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)

IHC staining of human kidney using 11916-1-AP (same clone as 11916-1-PBS)

IF Staining of A549 using 11916-1-AP (same clone as 11916-1-PBS)

FC experiment of HEK-293 using 11916-1-AP (same clone as 11916-1-PBS)

RABL3 Polyclonal antibody, PBS Only

RABL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™