Validation Data Gallery

Filter: AllWBFC (Intra)Affinity

WB analysis using 83470-3-RR (same clone as 83470-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83470-3-RR (RABL2B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.
FC experiment of A549 using 83470-3-RR (same clone as 83470-3-PBS)
1x10^6 A549 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.
×
1x10^6 A549 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.
FC experiment of MCF-7 using 83470-3-RR (same clone as 83470-3-PBS)
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.
×
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83470-3-RR
Biolayer interferometry (BLl) kinetic assays of 83470-3-RR against Human RABL2B were performed. The affinity constant is 0.256 nM.

RABL2B Recombinant monoclonal antibody, PBS Only

RABL2B Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83470-3-PBS

Clone No.240501D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

240501D3, Rab like protein 2B, Rab-like protein 2B

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

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MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83470-3-PBS targets RABL2B in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag2189 Product name: Recombinant human RABL2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC024281 Sequence: MAEDKTKPSELDQGKYDADDNVKIICLGDSAVGKSKLMERFLMDGFQPQQLSTYALTLYKHTATVDGRTILVDFWDTAGQERFQSMHASYYHKAHACIMVFDVQRKVTYRNLSTWYTELREFRPEIPCIVVANKIDADINVTQKSFNFAKKFSLPLYFVSAADGTNVVKLFNDAIRLAVSYKQNSQDFMDEIFQELENFSLEQEEEDVPDQEQSSSIETPSEEAASPHS 相同性解析による交差性が予測される生物種
Full Name	RAB, member of RAS oncogene family-like 2B
Calculated molecular weight	229 aa, 26 kDa
GenBank accession number	BC024281
Gene Symbol	RABL2B
Gene ID (NCBI)	11158
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q9UNT1
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 83470-3-RR (same clone as 83470-3-PBS)

FC experiment of A549 using 83470-3-RR (same clone as 83470-3-PBS)

FC experiment of MCF-7 using 83470-3-RR (same clone as 83470-3-PBS)

Affinity and Kinetic Characterization of 83470-3-RR

RABL2B Recombinant monoclonal antibody, PBS Only

RABL2B Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 83470-3-RR (same clone as 83470-3-PBS)

FC experiment of A549 using 83470-3-RR (same clone as 83470-3-PBS)

FC experiment of MCF-7 using 83470-3-RR (same clone as 83470-3-PBS)

Affinity and Kinetic Characterization of 83470-3-RR

RABL2B Recombinant monoclonal antibody, PBS Only

RABL2B Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

RABL2B Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA