Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells |
| Positive IHC detected in | human lung tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
14448-1-AP targets RABGGTA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5923 Product name: Recombinant human RABGGTA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 143-407 aa of BC003093 Sequence: FHCWDYRRFVATQAAVPPAEELAFTDSLITRNFSNYSSWHYRSCLLPQLHPQPDSGPQGRLPEDVLLKELELVQNAFFTDPNDQSAWFYHRWLLGRADPQDALRCLHVSRDEACLTVSFSRPLLVGSRMEILLLMVDDSPLIVEWRTPDGRNRPSHVWLCDLPAASLNDQLPQHTFRVIWTAGDVQKECVLLKGRQEGWCRDSTTDEQLFRCELSVEKSTVLQSELESCKELQELEPENKWCLLTIILLMRALDPLLYEKETLQY 相同性解析による交差性が予測される生物種 |
| Full Name | Rab geranylgeranyltransferase, alpha subunit |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 60~65 kDa |
| GenBank accession number | BC003093 |
| Gene Symbol | RABGGTA |
| Gene ID (NCBI) | 5875 |
| RRID | AB_10644135 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92696 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GGTase II (RAB geranylgeranyl transferase, RABGGTase) is a heterodimer composed of tightly bound α- and β-subunits, which belongs to the Ras superfamily of small monomeric GTPases. RABGGTase is extensively involved in intracellular membrane trafficking of membrane-bound organelles and vesicles and specifically catalyses the covalent attachment of GGPP to the cysteine of a carboxyl-terminal CC or CXC motif of RAB proteins (PMID 11136552, 10737774, 35961942).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RABGGTA antibody 14448-1-AP | Download protocol |
| IHC protocol for RABGGTA antibody 14448-1-AP | Download protocol |
| IF protocol for RABGGTA antibody 14448-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Pathog Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.
| ||
Cell Death Dis Simvastatin accelerated motoneurons death in SOD1G93A mice through inhibiting Rab7-mediated maturation of late autophagic vacuoles. | ||