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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RABGAP1 Polyclonal antibody (14223-1-AP)

    WB analysis using 14223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14223-1-AP (RABGAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RABGAP1 Polyclonal antibody (14223-1-AP)

    WB analysis using 14223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14223-1-AP (RABGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RABGAP1 Polyclonal antibody (14223-1-AP)

    WB analysis using 14223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14223-1-AP (RABGAP1 antibody) at dilution of 1:14000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using RABGAP1 Polyclonal antibody (14223-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 14223-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14223-1-AP (RABGAP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RABGAP1 Polyclonal antibody

RABGAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14223-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Rab6 GTPase-activating protein GAPCenA, Rab GTPase-activating protein 1, HSPC094, GAPCENA, GAP and centrosome-associated protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells, LNCaP cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14223-1-AP targets RABGAP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5462

Product name: Recombinant human RABGAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 697-997 aa of BC054492

Sequence: DDLLLTDFEGALKFFRVQLPKRYRSEENAKKLMELACNMKISQKKLKKYEKEYHTMREQQAQQEDPIERFERENRRLQEANMRLEQENDDLAHELVTSKIALRKDLDNAEEKADALNKELLMTKQKLIDAEEEKRRLEEESAQLKEMCRRELDKAESEIKKNSSIIGDYKQICSQLSERLEKQQTANKVEIEKIRQKVDDCERCREFFNKEGRVKGISSTKEVLDEDTDEEKETLKNQLREMELELAQTKLQLVEAECKIQDLEHHLGLALNEVQAAKKTWFNRTLSSIKTATGVQGKETC

相同性解析による交差性が予測される生物種
Full Name RAB GTPase activating protein 1
Calculated molecular weight 122 kDa
Observed molecular weight 121 kDa
GenBank accession numberBC054492
Gene Symbol RABGAP1
Gene ID (NCBI) 23637
RRIDAB_2878030
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3P9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RABGAP1 antibody 14223-1-APDownload protocol
IHC protocol for RABGAP1 antibody 14223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols