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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RABGAP1 Polyclonal antibody

RABGAP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14223-1-AP

Synonyms

GAPCENA, RABGAP1, RP11 123N4.2, TBC1D11

Validation Data Gallery

Applications:
  • WB analysis using 14223-1-AP

    WB analysis using 14223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14223-1-AP (RABGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14223-1-AP

    WB analysis using 14223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14223-1-AP (RABGAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14223-1-AP

    WB analysis using 14223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14223-1-AP (RABGAP1 antibody) at dilution of 1:14000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

14223-1-AP targets RABGAP1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABGAP1 fusion protein Ag5462 相同性解析による交差性が予測される生物種
Full Name RAB GTPase activating protein 1
Calculated molecular weight 122 kDa
Observed molecular weight 121 kDa
GenBank accession numberBC054492
Gene symbol RABGAP1
Gene ID (NCBI) 23637
RRIDAB_2878030
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RABGAP1 antibody 14223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols