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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis of mouse skin using 55295-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis using 55295-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis using 55295-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis of mouse colon using 55295-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis of human brain using 55295-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RAB8B Polyclonal antibody

RAB8B Polyclonal Antibody for WB, ELISA
Cat No. 55295-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RAB8, Ras related protein Rab 8B, Ras-related protein Rab-8B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skin tissue, human brain tissue, mouse colon tissue, rat skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55295-1-AP targets RAB8B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name RAB8B, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberNM_016530
Gene Symbol RAB8B
Gene ID (NCBI) 51762
RRIDAB_10896304
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.

Protocols

Product Specific Protocols
WB protocol for RAB8B antibody 55295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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