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RAB8A Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 39.1-2500 pg/mL

Cat no : MP02488-2

Print datasheet

Synonyms

EC:3.6.5.2, MEL, Oncogene c mel, Oncogene c-mel, RAB8

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02488-2, Human RAB8A Recombinant Matched Antibody Pair - PBS only. 86540-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30567. 86540-6-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

    Sandwich ELISA sample test of MP02488-2

    Sandwich ELISA standard curve of MP02488-2, Human RAB8A Recombinant Matched Antibody Pair - PBS only. 86540-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30567. 86540-6-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • The mean RAB8A concentration was determined to be 3.16 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 2.99 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load and 3.65 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

    Sandwich ELISA standard curve of MP02488-2

    The mean RAB8A concentration was determined to be 3.16 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 2.99 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load and 3.65 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02488-2 targets RAB8A in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: RAB8A Recombinant monoclonal antibody, PBS Only (Capture) 86540-7-PBS (251429C5). 100 μg. Concentration 1 mg/ml.

Detection antibody: RAB8A Recombinant monoclonal antibody, PBS Only (Detector) 86540-6-PBS (251429F7). 100 μg. Concentration 1 mg/ml.

Alternative RAB8A matched antibody pairs: MP02488-1

Matched pair protein standard: Ag30567

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86540-7-PBS
Tested Reactivity human
Immunogen RAB8A fusion protein Ag30567
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAB8A, member RAS oncogene family
Synonyms EC:3.6.5.2, MEL, Oncogene c mel, Oncogene c-mel, RAB8
GenBank accession numberNM_005370
Gene symbol RAB8A
UniprotP61006
Gene ID (NCBI) 4218
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86540-6-PBS
Tested Reactivity human
Immunogen RAB8A fusion protein Ag30567
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAB8A, member RAS oncogene family
Synonyms EC:3.6.5.2, MEL, Oncogene c mel, Oncogene c-mel, RAB8
GenBank accession numberNM_005370
Gene symbol RAB8A
UniprotP61006
Gene ID (NCBI) 4218
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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