• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Multi-rAb® RAB7A Multi-Recombinant antibody (RMX00029)

    WB analysis using RMX00029

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00029 (RAB7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using Multi-rAb® RAB7A Multi-Recombinant antibody (RMX00029)

    IP experiment of SH-SY5Y using RMX00029

    IP result of anti-RAB7A (IP:RMX00029, 4ug; Detection:RMX00029 1:5000) with SH-SY5Y cells lysate 1480 ug.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Multi-rAb® RAB7A Multi-Recombinant antibody (RMX00029)

    IP experiment of mouse brain using RMX00029

    IP result of anti-RAB7A (IP:RMX00029, 4ug; Detection:RMX00029 1:1000) with mouse brain tissue lysate 1960 ug.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Multi-rAb™ RAB7A Multi-Recombinant antibody (RMX00029)

    IHC staining of mouse kidney using RMX00029

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RMX00029 (RAB7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Multi-rAb™ RAB7A Multi-Recombinant antibody (RMX00029)

    IHC staining of mouse kidney using RMX00029

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RMX00029 (RAB7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Multi-rAb™ RAB7A Multi-Recombinant antibody (RMX00029)

    IF Staining of HeLa using RMX00029

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB7A antibody (RMX00029) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Multi-rAb® RAB7A Multi-Recombinant antibody

RAB7A Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. RMX00029

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RAB7, EC:3.6.5.2, Ras related protein Rab 7a, Ras-related protein Rab-7a

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue, SH-SY5Y cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00029 targets RAB7A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name RAB7A, member RAS oncogene family
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberNM_004637
Gene Symbol RAB7A
Gene ID (NCBI) 7879
Conjugate Unconjugated
Form
FormLiquid
Purification MethodN/A
UNIPROT IDP51149
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.

Protocols

Product Specific Protocols
IF protocol for RAB7A antibody RMX00029Download protocol
IHC protocol for RAB7A antibody RMX00029Download protocol
IP protocol for RAB7A antibody RMX00029Download protocol
WB protocol for RAB7A antibody RMX00029Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...