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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB5B Recombinant monoclonal antibody (86951-1-RR)

    WB analysis using 86951-1-RR (same clone as 86951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAB5B Recombinant monoclonal antibody (86951-1-RR)

    WB analysis using 86951-1-RR (same clone as 86951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAB5B Recombinant monoclonal antibody (86951-1-RR)

    WB analysis using 86951-1-RR (same clone as 86951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAB5B Recombinant monoclonal antibody (86951-1-RR)

    WB analysis using 86951-1-RR (same clone as 86951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAB5B Recombinant monoclonal antibody (86951-1-RR)

    WB analysis using 86951-1-RR (same clone as 86951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAB5B Recombinant monoclonal antibody (86951-1-RR)

    WB analysis using 86951-1-RR (same clone as 86951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86951-1-RR (RAB5B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86951-1-PBS in a different storage buffer formulation.

  • Affinity of 86951-1-RR

    Affinity of 86951-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86951-1-RR against Human RAB5B were performed. The affinity constant is 0.668 nM.

RAB5B Recombinant monoclonal antibody, PBS Only

RAB5B Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86951-1-PBS
Clone No.252038B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

EC:3.6.5.2, Ras related protein Rab 5B, Ras-related protein Rab-5B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86951-1-PBS targets RAB5B in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg5609

Product name: Recombinant human RAB5B protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 1-215 aa of NM_001252036.2

Sequence: MTSRSTARPNGQPQASKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQETFARAKTWVKELQRQASPSIVIALAGNKADLANKRMVEYEEAQAYADDNSLLFMETSAKTAMNVNDLFLAIAKKLPKSEPQNLGGAAGRSRGVDLHEQSQQNKSQCCSN

相同性解析による交差性が予測される生物種
Full Name RAB5B, member RAS oncogene family
Calculated molecular weight24 kDa
Observed molecular weight 24-27 kDa
GenBank accession numberNM_001252036.2
Gene Symbol RAB5B
Gene ID (NCBI) 5869
RRIDAB_3745229
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP61020-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAB5B (Ras-related protein Rab-5B) belongs to the Rab family. Rab5 is specifically localized to the early compartment and regulates endocytosis (PMID:1516130, PMID:9087439). There are three isoforms of Rab5 namely, Rab5a, Rab5b, and Rab5c present in EE of mammalian cells (PMID:7789520). Recently, we have shown that Rab5a regulates fluid-phase endocytosis whereas receptor-mediated endocytosis is regulated by Rab5b in Leishmania (PMID:27226564).

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